Figure 3

Heatmap representation of expression of the FOXM1 regulatory network in OAC patient samples. Heatmap summary of Nanostring nCounter gene expression analysis of 49 direct FOXM1 target genes (bottom panel) or genes encoding members of the MMB and DREAM complex (top panel), in samples from normal and tumour tissues. The expression level of each gene is represented by the z-score of the normalised mRNA level across all samples. The mRNA levels were normalised by the geometric mean of the GAPDH, ALAS1, PARPBP, HMBS and SDHA internal reference genes. Blue and cream represent high and low expression respectively as indicated by the scale bar. Rows (representing individual genes) and columns (representing individual tissue samples from normal (NRML) and tumour (OAC) tissues) are ordered by unsupervised hierarchical clustering of the 49 FOXM1 target genes. Major clusters of genes are indicated (A-C) and samples are broadly categorised into clusters of normal or tumour samples. Genes bound by FOXM1 in both OE33 and U2OS cells are marked by an orange dot. The position of FOXM1 is indicated by the black arrow. Clinical information on overall (AJCC) tumour stage (early/late), T stage (early/late) and presence or absence of nodal or distant metastasis is shown for each tumour sample above the heatmap (top four rows, coloured boxes). Darker coloured boxes represent late T/AJCC stage or presence of nodal/distant metastasis and lighter coloured boxes represent early T/AJCC stage or absence of nodal/distant metastasis as is indicated by the legend. Grey dots indicate samples from normal oesophageal tissue.