Increased DNA methylation and increased sensitivity to DNA demethylation of ARG72 carrying HCC cell lines. (A) MSRE-coupled qPCR analysis following sequential treatment of the cells by decitabine (5 μM/72 h) and doxorubicin (0.25 μM/24 h). ARG/ARG cell lines are often more resistant to methylation-sensitive DNA restriction than PRO/PRO cells that usually yield weaker signal in qPCR. The figure represents the outcome of two independent experiments in triplicate. Fold is relative to decitabine-treated only DNA. (B) Cell viability analysis of Cell lines as measured by MTT assay in different treatment conditions.