Figure 5

MRI, H & E staining and immunohistochemistry data of the patient and mouse’s brain tissue. A1, A2: Pre-surgery contrast-enhanced MRI images of T1WI and T2WI sequences of the patient; A3: Patient’s pathology H & E staining, light microscopy (×400); A4-A9: Glioma tissue immunohistochemistry: A2B5 (+ + +), GFAP (+ +), VEGFR (+ +), S-100 (+ +), VEGF (+ + +), and Ki-67 (+) (×400); B1-B2: H & E staing of intracranial xenograft of SHG-139, light microscope (×100, ×400); B3-B4: GFAP and A2B5 staining of intracranial xenograft of SHG-139, light microscope (×1000); C1-C3: HE of intracranial xenograft of SHG-139S, C1: Low magnification microscopy showed invasive growth of tumor cells (×100), C2: High magnification microscopy showed local area in the xenograft (×400), C3: “Fried egg-like” cells-based high cell density areas were visible in another area of the xenograft (×400); D1-D9: Immunohistochemistry of intracranial xenograft of SHG-139S: D1-D2: Expression of intratumor and peritumoral A2B5 (+) (×400); D3: Expression of CD133 in the xenograft (+) (×400); D4, D6: Expression of GFAP (+ +) and S-100 (+ +) in the xenograft (×400); D5, D7: Expression of GFAP (+ + +) and S-100 (+ + +) intratumor oligodendroglioma components in the xenograft (×400); D8, D9:0020cxxCD31 (+) and CD34 (+) staining (×400).