miR-320a inhibits the invasiveness of SACC cells by targeting ITGB3. (A) Integrin β3 expression and FAK and Src phosphorylation were determined by western blot analysis in ACCM cells transfected with miR-320a mimics alone or in combination with either pcDNA 3.1+ (Vector) or pcDNA 3.1+ containing a wild-type (ITGB3) or mutant ITGB3 expression cassette of the miR-320a (ITGB3 mut) response element. β-actin was used as an internal control. Adhesion, invasion and migration of ACCM cells were assessed using adhesion assays (B) and transwell assays (C, D) (100x). (E) Quantification of adhesive, invasive and migratory ACCM cells. *P < 0.05; **P < 0.01; ***P < 0.001 vs. vector.