Figure 4

Ectopic expression of miR-320a inhibits ACCM xenograft metastasis in BALB/c-nu mice. (A) Whole-body luminal imaging was performed in mice inoculated with ACCM stably expressing the luciferase gene. Luminal images (B) and HE staining (C) of paraffin sections (200×) of the lungs (upper) and livers (lower) of the tumor-bearing mice. (D) Mean ± SD wet lung weight of tumor-bearing mice (n = 8/group). (E) Expression of human HPRT mRNA relative to mouse 18S rRNA in the lungs and livers of the tumor-bearing mice was determined by qRT-PCR analysis. **P < 0.01 vs. ACC2 cells. ^## P < 0.01 vs. EmGFP transfection.