Figure 4

RU-SKI 43 does not alter localization or activation of ERα. A, MCF7 cells were cultured in the presence of DSMO or 10 μM RU-SKI 43 for 4 h. Cells were fixed and stained with anti-ERα. Three independent experiments were performed using cells at three different passages. B-C, MCF7 (B) or TamR (C) cells were treated with DSMO or 10 μM RU-SKI 43 for 4 h. Cells were treated with ethanol or 17β-estradiol for 30 minutes prior to lysis. Cell lysates were analyzed directly by Western blotting with indicated antibodies. Three independent experiments were performed in duplicate using cells at three different passages.