Molecular analysis from untreated mammary glands and DMBA-induced mammary tumours. (A) Representative protein expression changes within untreated mammary glands (MG) and in vivo generated mammary tumours in DMBA Only- and DMBA + ROSI-treated groups were analyzed by Western Blot as described in the Methods section. PPARγ, Cox-2 and PTEN protein levels were analyzed in untreated virgin MG from PPARγ-WT (WT) and PPARγ-MG KO (MG KO) mice, as well as all available breast tumour subtypes from both strains of mice. β-actin served as loading control. (B) Densitometry for PPARγ, Cox-2 and PTEN were performed on all mammary tumours using ImageJ software, and expressed as mean ± SD. Fold changes are relative to mammary tissue from untreated PPARγ-WT. Black bars, PPARγ expression; white bars, Cox-2 expression; grey bars, PTEN expression; **, p < 0.01; ****, p < 0.0001.