Reinforced FLJ10540 expression resists the cytotoxicity to cisplatin in depletion and inactivation cells of Aurora-A. (A) SAS/negative control or SAS/siAurora-A cells co-transfected with vehicle or FLJ10540 were incubated with increasing concentrations of cisplatin for 48 hour, and their percentage viability was measured and compared to that of untreated respective cells. (B) The cells were cultured in the presence of cisplatin (15 μM) for 48 hour, and their viability was measured. Vehicle and FLJ10540 transfectants were cultured in the presence or absence of cisplatin (15 μM) and/or MLN8237 for 48 hour (C) or 15 days (D) and total cell viability were assessed by MTT assay and soft agar. Statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001.