Figure 6

Aurora-A modulates MMP-7 and MMP-10 expression and secretion in HNC cells. The mRNA expression levels of MMP-1, −2, −3, −7, −9, −10, −12, −13 and −14 (A) and protein expression profiles of MMP-7 and −10 (B) were examined by Q-RT-PCR and Western blotting in SAS/negative control and SAS/siAurora-A transfectants. (C and D) The endogenous mRNA and protein expression levels of MMP-7, and −10 (C) were examined by Q-RT-PCR and Western blotting in FaDu/vehicle and FaDu/Aurora-A transfectants. (E) Unsynchronized Aurora-A transfectants were fixed, and immunostained with antibodies against endogenous MMP-7 and −10 or DAPI. Representative data are shown. (F) Using the Figure 4F xenograft model. The paraffin-embedded tumor tissues were subjected to immunostainings for MMP-7 and MMP-10. (G) The migration and invasion assays were performed by Transwell chambers in Aurora-A transfectants treated with GM6001 (3 μM). D: DMSO; G: GM6001. Statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001.