Figure 7

FLJ10540 not only promotes MMP-7 and −10 expressions through enforced Aurora-A expression, but also has a highly correlation with Aurora-A, MMP-7 and −10 expressions in human HNC tissues. FLJ10540 induced an increase in MMP-7, and −10 mRNA (A) and protein (B, left panel) levels in FaDu/FLJ10540 transfectants. (B, right panel) Conditioned media were prepared by incubating FaDu/vehicle and FaDu/FLJ10540 transfectants in serum-free media for 24 hour. MMP-7 and −10 in the samples were analyzed by ELISA. (C and D) The mRNA and protein expression profiles of MMP-7 and −10 were examined by Q-RT-PCR and Western blotting in SAS/negative control and SAS/siFLJ10540 transfectants. (E) The MMP-7 and −10 expression levels in conditioned media of vehicle and Aurora-A cotransfected with negative control or siFLJ10540 were assay by ELISA. (F) The FLJ10540, Aurora-A, MMP-7 and MMP-10 expressions in human HNC specimens were investigated by immunohistochemical staining. Photographs of weak and strong staining for FLJ10540, Aurora-A, MMP-7 and MMP-10 in the sections are shown. Statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001.