Figure 1

miRNA expression profiling in HepG2 cells incubated under normoxia or hypoxia with or without etoposide. Relative expression of 132 miRNAs was quantified by TLDA (TaqMan Low Density Array) in cells incubated under normoxia (N) or hypoxia (H) with or without etoposide (E) (50 μM) during 16 h. (A) Ratio of miRNA expression between cells incubated with etoposide under hypoxia in comparison to normoxia has been calculated. Four miRNAs were detected with an induction fold higher than 1.5 and seven miRNAs with a fold induction lower than - 1.5. Data were normalized to RNU44 and RNU48 endogenous controls using the threshold cycle method. (B) miRNA-565, −196b and −210 expression was monitored by conventional TaqMan RT-qPCR in HepG2 cells exposed to normoxia (N) or hypoxia (H) with or without etoposide (E) (50 μM) during 16 h. The relative expression of miRNA was determined using the threshold cycle method by normalizing to endogenous RNU44 (means ± 1SD, n = 3). ***: significantly different from normoxia (p < 0.001), #, ###: significantly different from hypoxia (p < 0.05, p < 0.001), $$$: significantly different from normoxia with etoposide (p < 0.001).