Proteomic analysis after miR-196b overexpression in HepG2 cells. HepG2 were transfected with pre-miR negative control or pre-miR-196b (50 nM) during 24 h and 2D-DIGE analysis of cytosolic fraction was performed (n = 4). (A) Proteins were loaded on IPG strips (18 cm, 3–11 non linear), followed by the second dimension on a vertical continuous 12.5% gel. Significantly down- or up-regulated proteins (cut-off 1.2, p value < 0.05) were identified and labeled with their serial number. Arrow: spot 952/942, IGF2BP1 protein. Table shows the number of down-regulated or up-regulated spots. (B) List of proteins identified by MS/MS analysis and predicted by softwares: TargetScan, MicroCosm and miRDB. (C) Intensity of spot 952 was significantly lower when HepG2 cells were transfected with pre-miR-196b versus pre-miR negative control during 24 h. Dot plots showed the variation between pre-miR negative control and pre-miR-196b for 4 replicates.