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Figure 5 | Molecular Cancer

Figure 5

From: miRNA-196b inhibits cell proliferation and induces apoptosis in HepG2 cells by targeting IGF2BP1

Figure 5

Effects of miR-196b overexpression or IGF2BP1 silencing on cell morphology, viability and proliferation. HepG2 cells were transfected either with pre-miR negative control (pre-miR CTL-) or pre-miR-196b (50 nM) either with RISC Free negative control (RF) or IGF2BP1 siRNA (50 nM). (A) 72 h after transfection, the cell morphology was observed by phase contrast microscopy. (B) Cell viability assay (MTT assay) was performed 24, 48, 72 and 96 h after transfection of HepG2 cells (means ± 1 SD, n = 3). (C) Cell proliferation assay (BrdU ELISA assay) was performed 72 h after transfection (means ± 1 SD, n = 3). °°, °°°: significantly different from untransfected cells (Cells) (p < 0.01, p < 0.001), $$, $$$: significantly different from pre-miR negative control transfected cells or RF negative control transfected cells (p < 0.01, p < 0.001).

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