Effects of miR-196b overexpression or IGF2BP1 silencing in regulation of apoptosis under normoxia. (A) HepG2 cells were transfected with either pre-miR negative control (pre-miR CTL-) or pre-miR-196b (50 nM), either with RISC free negative control (RF) or siRNA IGF2BP1 for 24, 48 and 72 h. IGF2BP1, cleaved PARP and cleaved caspase 3 were detected in total cell extracts by western blotting analysis, using specific antibodies. Immunodetection of α-tubulin was used to assess the total amount of proteins loaded on the gel. Numbers correspond to the quantification of the abundance of protein of interest normalized to the abundance of α-tubulin. (B) 48 h after transfection, the caspase 3/7 activity was assayed by measuring free AFC released from the cleavage of the caspase 3/7 substrate Ac-DEVD-AFC. Results are expressed in fluorescence intensity, as means ± 1 SD (n = 3). °, °°°: significantly different from untransfected cells (Cells) (p < 0.05, p < 0.001), $$$: significantly different from pre-miR negative control transfected cells (p < 0.001).