Figure 7

Effects of miR-196b overexpression or IGF2BP1 silencing on c-myc mRNA expression and protein level. (A) HepG2 cells were transfected with RISC free negative control (RF) or siRNA IGF2BP1 (50 nM) during 24 or 48 h and the mRNA expression level of IGF2BP1 was determined by RT-qPCR (means ± 1 SD, n = 3). °, °°, °°°: significantly different from untransfected cells (Cells) (p < 0.05, p < 0.01, p < 0.001), $$$: significantly different from RF negative control transfected cells (p < 0.001) for each group 24 or 48 h respectively. (B) HepG2 cells were transfected with RISC free negative control (RF) or siRNA IGF2BP1 (50 nM) during 24, 48 and 72 h and the mRNA expression level and the protein abundance of c-myc were determined by RT-qPCR and western blot respectively. (C) HepG2 cells were transfected with pre-miR negative control (pre-miR CTL-) or pre-miR-196b (50 nM), during 24, 48 and 72 h and the mRNA expression level and the protein abundance of c-myc were determined by RT-qPCR and western blot respectively. Numbers correspond to the quantification of the abundance of protein of interest normalized to the abundance of α-tubulin.