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Figure 8 | Molecular Cancer

Figure 8

From: miRNA-196b inhibits cell proliferation and induces apoptosis in HepG2 cells by targeting IGF2BP1

Figure 8

Role of IGF2BP1 in the chemoresistance induced by hypoxia. HepG2 cells were transfected with RISC free negative control (RF) or siRNA IGF2BP1 (50 nM) during 48 h and then cells were incubated under normoxia (N) or hypoxia (H) with or without etoposide (E) (50 μM) during 16 h. (A) IGF2BP1, cleaved caspase 3 and cleaved PARP were detected in total cell extracts by western blotting analysis, using specific antibodies. Immunodetection of α-tubulin was used to assess the total amount of proteins loaded on the gel. Numbers correspond to the quantification of the abundance of protein of interest normalized to the abundance of α-tubulin (B) 48 h after transfection, the caspase 3/7 activity was assayed by measuring free AFC released from the cleavage of the caspase 3/7 substrate Ac-DEVD-AFC. Results are expressed in fluorescence intensity, as means ± 1 SD (n = 3). ***: (p < 0.001), °, °°°: significantly different from untransfected cells (Cells) (p < 0.05, p < 0.001), $$$: significantly different from pre-miR negative control transfected cells (p < 0.001) for each group N, H, NE, HE respectively.

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