Involvement of mTOR in the synergistic activation of
. a) Western blot analysis of mTOR phosphorylation levels in OVSAYO cells cultured under the indicated conditions for 16 h. The phosphorylation levels shown were based on band intensity quantification using ImageJ software. b) Effect of rapamycin on the synergistic activation of ICAM1 in cells cultured under SSH for 16 h. Data shown are the mean (n = 2) ± SD. c) Western blot analysis of HIF expression in OVSAYO cells cultured under SSH for 16 h in the presence or absence of rapamycin. d) Effect of mTOR on the synergistic ICAM1 activation in OVSAYO cells transfected with siRNAs. Data shown are the mean (n = 3) ± SD. *P = 0.019, compared with the vehicle control. Inset: western blot analysis of ICAM1 expression in cells transfected with the indicated siRNAs and cultured under SSH for 24 h. e) Effects of mTOR on the expression levels of HIFs in OVSAYO cells cultured as indicated. f) Effects of mTOR on mRNA levels of KLF6 and JUN in OVSAYO cells. Cells were transfected with siRNAs as indicted and cultured for 24 h. Cells were further cultured as indicated for 16 h. Data shown are the mean (n = 2) ± SD.