Enhanced apoptotic and reduced migratory and invasive ability of Raf265 by targeting on CD26+ CSCs. A. CD26+ and CD26− cells were treated with different combinations of Raf265 and 5FU and the percentage of annexin V positive cells were analyzed. After treatment, representing view under 100× magnification of the B. migrated and C. invaded cells were shown at the upper panel and the average numbers of the B. migrated and C. invaded cells were plotted and shown at the lower panel. Data are presented as means ± SD from three independent experiments and statistical analysis was performed by one-way ANOVA. *p < 0.05 versus untreated control, **p < 0.05 versus 0 μM Raf265 and 10 μM or 5 μM 5FU as indicated and # p < 0.05 versus CTL cells. ***p < 0.05 versus 1 μM Raf265 and 0 μM 5FU.