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Figure 4 | Molecular Cancer

Figure 4

From: Long noncoding RNA PVT1 indicates a poor prognosis of gastric cancer and promotes cell proliferation through epigenetically regulating p15 and p16

Figure 4

Subcellular fractionation location of PVT1, and PVT1 could bind to EZH2. (A) After nuclear and cytosolic separation, RNA was extracted from the nuclear and the cytoplasmic fraction of SGC-7901 and BGC-823 cells and PVT1 expression was measured by qRT-PCR. GAPDH was used as a cytosol marker and U6 was used as a nucleus marker. (B) RIP experiments were performed in SGC-7901 and BGC-823 cells and the coprecipitated RNA were subjected to qRT-PCR for PVT1. HOTAIR was used as a positive control. The fold enrichment of PVT1 in EZH2 RIP is relative to its matching IgG control RIP. *, P < 0.05, **, P < 0.01.

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Molecular Cancer

ISSN: 1476-4598