Subcellular fractionation location of PVT1, and PVT1 could bind to EZH2. (A) After nuclear and cytosolic separation, RNA was extracted from the nuclear and the cytoplasmic fraction of SGC-7901 and BGC-823 cells and PVT1 expression was measured by qRT-PCR. GAPDH was used as a cytosol marker and U6 was used as a nucleus marker. (B) RIP experiments were performed in SGC-7901 and BGC-823 cells and the coprecipitated RNA were subjected to qRT-PCR for PVT1. HOTAIR was used as a positive control. The fold enrichment of PVT1 in EZH2 RIP is relative to its matching IgG control RIP. *, P < 0.05, **, P < 0.01.