PP4C promoted CRC cell tumorigenicity and invasiveness of SW480 cells in vivo. (A) Representative photographs of tumors derived from SW480-PP4C or SW480-vector control cells in nude mice. The bar graphs showed the average tumor weight (gram). (B) At the end of the experiment, the expression of proteins of PP4C, MMP-2 and MMP-9 were determined in tissues from mice bearing SW480 xenograft tumors. (C) The number of tumor nodules and foci in the lungs was counted based on hematoxylin and eosin staining (Original magnification, 200 × for B). The values represent the means ± SD from 18 mice. **P < 0.01 (Student’s t test).