PP4C regulated CRC cell proliferation and invasion. (A) Western blotting analysis of PP4C in SW480 and HT29 cells transfected with PP4C or a vector control. (B, C) Effect of PP4C overexpression on cell proliferation was evaluated by CCK-8 assay [(B) SW480 and (C) HT29]. (D) Representative micrographs of invasion assay of the indicated cells at 24 h. (E) Western blotting analysis of PP4C in SW620 and LOVO cells transfected with shPP4C or a negative control. (F, G) Proliferation rates of cell sublines detected by CCK-8 assay after PP4C knockdown [(F) SW480 and (G) HT29]. (H) Representative micrographs of the indicated cells grown for 24 h on Matrigel in the invasion assay (Original magnification, 100× for D and H). The data are shown as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01.