Re-expression of miRNA induces downregulation of TCF4. A) RT-qPCR of miRNA isolated from MiaPaCa cells 24 h after transfection with PCMV-MIR-802 or PCMV-MIR-Control. miRNA-802 expression: fold-change with standard deviation (n = 3). B) TCF4 expression was analyzed 24 h after expression of PCMV-MIR-802 and PCMV-MIR-Control in MiaPaCa cells. Β-Actin was used as a loading control and for normalization (n = 3). C) LI-COR quantification of TCF4 protein levels in miRNA-treated cells. TCF4 levels were normalized to β-Actin (n = 3).