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Figure 2 | Molecular Cancer

Figure 2

From: FL118, a novel camptothecin derivative, is insensitive to ABCG2 expression and shows improved efficacy in comparison with irinotecan in colon and lung cancer models with ABCG2-induced resistance

Figure 2

Genetic silencing or overexpression of ABCG2 demonstrates that the potency of FL118 is not affected by ABCG2. A and D, Western blot analysis of ABCG2 protein expression in HCT116-A2 cells that were stably transduced with a non-silencing shRNA control (ns) or anti-ABCG2 shRNA (sh1 = V3LHS_380805, sh2 = V3LHS_380806) (A), and HEK293 cells that were stably transfected with either an ABCG2 expression vector (HEK293/ABCG2) or a corresponding empty vector (HEK293/pcDNA3) (D). B and C, dose-response curves of SN-38 (B) and FL118 (C) after 72-hour treatments in HCT116-A2 cell lines. E and F, dose-response curves of SN-38 (E) and FL118 (F) after 72-hour treatments in HEK293 cell lines that were stably transfected with ABCG2 expression vector or empty vector. Viability was determined as in Figure 1. Error bars = SEM, n = 3 independent experiments.

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