Figure 6

Detecting differential levels of KIT protein. A: Western blot analysis for KIT protein in K562, Vec (GER vector transformed), 3–4, 22B11, 3D1 and 32H8 cells. Arrow and asterisks indicate 145 kD membrane bound KIT (arrow), a 120 kD form of KIT (*) and a 100 kD (**) soluble form of KIT. The same blot reprobed with an antibody for beta actin as a loading control is shown below. B: Immunocytochemistry for KIT in K562, Vec and KD clones (3–4 and 22B11). Nuclei are counter-stained with Hoechst 33342 (blue). KIT protein is stained red. Higher magnifications for 3–4 and 22B11 are also shown. Scale bars, 50 μm.