Klf5 deletion increases blood microvessels in mouse prostate tumors. H&E staining and IHC staining of Cd31 for blood microvessels from prostates at 8 months for Pten
(A) and 12 to 18 months for Pten
(B). Black arrows point to microvessels inside prostatic acini. Images at the right upper corner are higher magnifications of boxed areas. Microvessels were counted in dorsal lobes for Pten
−/− prostates and the entire lobes for Pten
+/− prostates. The total area of prostatic epithelia excluding the lumen space inside the acini was measured with ImageJ software. Gene status is indicated at the top of each panel, and + and – indicate wildtype and deletion of Klf5 or Pten, respectively. Three to 6 mice were used for each group. Microvessel density was defined by the number of microvessels per mm2. *, P < 0.05; **, P < 0.01; N.S., not statistically significant.