Figure 2

Klf5 deletion dysregulates angiogenesis-related genes and enhances HIF1α transcriptional activity in Pten-null mouse prostates. (A) Heatmap of genes involved in angiogenesis based on the MetaCore analysis and PubMed publications and that had >1.5 fold change in expression between wildtype and Klf5-null mouse prostates (4 samples per group, indicated at the top). Genes are clustered by their effects on angiogenesis, with those promoting and repressing angiogenesis marked as “promote” and “repress”, respectively. Color intensities in the heat map represent log2 values of expression intensities. The percentage of upregulated or downregulated genes is shown. (B) HIF1α is activated by Klf5 deletion, as indicated by the upregulation of its direct transcriptional target genes based on MetaCore’s interactome analysis for transcription factors. Different shapes of the nodes represent functional classifications of genes. Red dots on the upper right corner of each symbol indicate increased expression level, and green lines indicate positive regulation. (C) Detection of Hif1α/Vegf and Pdgf signaling molecules by real-time RT-PCR in 6-month-old Pten-null mouse dorsal prostates with indicated Klf5 deletion status. Data for each genotype were from 8 mice.* and **indicate P < 0.05 and P < 0.01, respectively.