Klf5 deletion increases protein expression of Hif1α and pro-angiogenic factors. (A, B) IHC staining of Hif1α in mouse prostates at 4 months for Pten
−/− and 12 months for Pten
+/−. The numbers of Hif1α-positive cells and total prostatic epithelial cells were counted with ImageJ software for each mouse. (C, D) Protein expression of pro-angiogenic factors Vegf, Pdgf-d and Pdgf-b as detected by IHC staining with the same mouse tissues used in panels A and B. Three or 4 mice were used for each group. *, P < 0.05; **, P < 0.01; N.S., not statistically significant.