Figure 5

JAKi increased the anti-tumor activity of gefitinib in mice. (A) SKOV3 cells were implanted subcutaneously into the right flank of nude mice. Tumors were treated with vehicle, JAKi (30 mg/kg), gefitinib (150 mg/kg) or their combination by oral gavage once daily. Tumor growth was measured twice a week. Data represents means ± SD (n = 8-10). *, P < 0.05; ***, P < 0.005, combination vs. vehicle, or gefitinib alone or JAKi alone. (B) Western blots show the effects of treatment on the indicated intracellular signaling pathways in tumor tissue. (C) Relative levels of p-EGFR, p-STAT3, p-ERK were determined by measuring the density of each band and normalized to that of GAPDH. Densitometry data were relative changes in protein expression and were mean ± SD of 2–3 preparations. *, P < 0.05; ***, P < 0.0005.