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Figure 8 | Molecular Cancer

Figure 8

From: X-shaped DNA potentiates therapeutic efficacy in colitis-associated colon cancer through dual activation of TLR9 and inflammasomes

Figure 8

XL-DNA and XS-DNA induce inflammasome activation. A, B. After BMDCs were primed with LPS (10 ng/ml, 18 h), cells were transfected with XL- DNA, XS-DNA, or poly dA:dT for (A) 6 h or (B) 18 h. C, D. After BMDCs from wild-type (WT) or caspase-1-knockout (Casp1KO) mice were primed with LPS (10 ng/ml, 18 h), cells were transfected with X-DNA (2 μg) or poly dA:dT (1 μg) for (C) 6 h or (D) 18 h. ATP (5 mM) was added to dendritic cells primed with LPS for 1 h (C) or 18 h (D). For A and C, supernatant proteins and whole cell lysates (WCL) were loaded on gels and subjected to SDS-PAGE. For B and D, concentration of IL-1β was measured by ELISA. Values are mean ± SEM (n = 3). *, p < 0.05. Representative data from three independent experiments are presented.

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