Figure 1
![Figure 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12943-015-0370-9/MediaObjects/12943_2015_370_Fig1_HTML.gif)
Restin expression level was negatively related with tumor cell mobility. (A) Restin overexpression failed to inhibit cell proliferation determined by [3H] thymidine incorporation assay. MCF-7 and MDA-MB-231 (2 x 104) cells were seeded onto 24-well plates. [3H] thymidine (1 mCi/well) was added 4 hours before the termination of experiment. Data were represented as the means ± S.M. of three independent experiments. mRNA (B) and protein (C) levels of Restin were determined by RT-PCR and western blot. MCF-10A, HMEC, MCF-7, MDA-MB-231 and MDA-MB-451 cells (1 x 105) were seeded onto 6-well plates. Values were expressed as means ± S.M. of at least three independent experiments. (D) Restin expression in MCF-7 cells upon TGF-β treatment was determined by western blot. MCF-7 cells (5 x 104) were seeded onto 6-well plates and TGF-β (5 ng/ml) were added for 6 days. Tubulin was used as a loading control. Results presented here are representative of three different experiments.