Cell type dependent induction of an aggressive phenotype in VM-CUB1 cells. (a) Phenotypical changes of stably transfected VM-CUB1 and 5637 clones as seen in light microscopy (10x magnification). (b) Immunofluorescence stainings for E-Cadherin (top, green) and Vimentin (bottom, green) to verify EMT induction in VM-CUB1-HOTAIR 20 cells (40 x magnification). Transwell assays were performed to determine changes in migration (c) and invasion (d, e) capacity (**p ≤ 0.001). Data results from one assay with 4 transwells per cell line and 5 visual fields, which were randomly chosen for counting the number of migrated or invaded cells. Results are representative for assays repeated in triplicate. Invaded cells were visualized by H&E staining (d, 10 x magnification).