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Figure 1 | Molecular Cancer

Figure 1

From: Impaired autophagy in mouse embryonic fibroblasts null for Krüppel-like Factor 4 promotes DNA damage and increases apoptosis upon serum starvation

Figure 1

MEFs null for Klf4 exhibit lower levels of autophagy compared to the wild-type during starvation. (A) Morphology of Klf4 +/+ and Klf4 −/− MEFs with time course EBSS treatment. Klf4 +/+ and Klf4 −/− MEFs were treated with EBSS for the durations indicated and photographed with an Olympus IX51 inverted microscope. Cell lysates from Klf4 +/+ (B) and Klf4 −/− (C) MEFs were analyzed using Western blot. Antibodies against LC3B were used to indicate autophagy levels and β-Actin was used as a loading control. (D) Densitometry analysis of LC3B bands shown in (B) and (C). The intensities of LC3BII bands were normalized to that of β-actin using Gel Imager program. Error bars represent standard error. The experiments were repeated 3 times. * indicates p < 0.05, ** indicates p < 0.01.

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Molecular Cancer

ISSN: 1476-4598