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Figure 2 | Molecular Cancer

Figure 2

From: Impaired autophagy in mouse embryonic fibroblasts null for Krüppel-like Factor 4 promotes DNA damage and increases apoptosis upon serum starvation

Figure 2

Impairment of autophagy in Klf4 −/− MEFs leads to increased apoptotic cell death. (A) Morphology of Klf4 +/+ and Klf4 −/− MEFs treated with full media, 10 μM CQ, EBSS, or both CQ and EBSS for 8 hrs. (B) MEFs were collected after respective treatments and subjected to Trypan Blue assay. The percentage of dead cells, as indicated by Trypan Blue staining, was quantified using a hemocytometer under bright field microscopy. Error bars represent standard deviation. The experiments were repeated 3 times. * indicates p < 0.05. (C) Klf4 +/+ and Klf4 −/− MEFs were treated with full media, 10 μM CQ, EBSS, or both CQ and EBSS for 8 hrs before the cells were analyzed using flow cytometry. Representative results from three independent experiments were shown. Sub-G1 population was identified for quantification of cell survival. (D) Sub-G1 population from the three independent experiments represented in (C) was averaged and plotted. Error bars represent standard error. * indicates p < 0.05.

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