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Figure 3 | Molecular Cancer

Figure 3

From: Impaired autophagy in mouse embryonic fibroblasts null for Krüppel-like Factor 4 promotes DNA damage and increases apoptosis upon serum starvation

Figure 3

Impaired autophagy in Klf4 −/− MEFs results in increased DNA damage. Klf4 +/+ and Klf4 −/− MEFs were treated with full-media or EBSS for 8 hrs. (A) Immunostaining against γ-H2AX (green), a DNA damage marker, was performed to assess the levels of DNA damage in Klf4 +/+ and Klf4 −/− MEFs. DAPI (blue) was used to visualize the nuclei. (B) The percentage of Klf4 +/+ and Klf4 −/− MEFs cultured in full-media or EBSS with less than 5, 5—15, or more than 15 γ-H2AX foci was quantified. At least 300 MEFs were counted for each condition. * indicates p < 0.05, ** indicates p < 0.01. (C) Western blot analysis was performed to assay the levels of γ-H2AX. The experiments were repeated 3 times.

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Molecular Cancer

ISSN: 1476-4598