Figure 4

The effects of dasatinib and methyl β-cyclodextrin on the activation of JNK by POH in U87 cells. Cells were pretreated with 10 nM dasatinib and 7.5 mM methyl β-cyclodextrin for 30 minutes. After the pretreatment, DMSO (0.1%) or POH (1.5 mM) was added. After 30 minutes incubation, the expression of p-JNK1/2 and total JNK1/2 were detected by western blot. The data were expressed as the means ± SD from at least three different experiments. **p < 0.01 vs. control group (0.1% DMSO). ††p < 0.01, ‡‡p < 0.01 vs. control group (1.5 mM POH without pretreatment), analyzed by Student’s t-test.