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Figure 6 | Molecular Cancer

Figure 6

From: Na/K-ATPase as a target for anticancer drugs: studies with perillyl alcohol

Figure 6

The effects of JNK inhibition on the induction of cell death by POH in U87 cells. Before treatment, U87 cells were incubated without (A and B) or with (C and D) JNK inhibitor V (0.5 μM) for 30 minutes. The cells were treated with 0.1% DMSO (A), 0.5 mM POH (B) 0.1% DMSO plus JNK inhibitor V (C), and 0.5 mM POH plus JNK inhibitor V (D). After 24 hours of incubation, the cells were stained with annexin V-FITC and propidium iodide and analyzed by flow cytometry. Figure 6E represents the percentage of dead cells indicated by early apoptosis and late apoptosis or necrosis (right lower quadrant + right upper quadrant, respectively), which was calculated from the data shown in Figures 6A-D. The data were expressed as the means ± SD from at least three different experiments. ***p < 0.001 vs. control group (0.1% DMSO), **p < 0.01 vs. control group (0.1% DMSO + JNK inhibitor V), †p < 0.05 vs. control group (0.5 mM POH without the JNK inhibitor V), analyzed by Student’s t-test.

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