Figure 7

The effects of JNK inhibition on the induction of cell death by POH in U251 cells. Before treatment, U251 cells were incubated without (A and B) or with (C and D) JNK inhibitor V (0.5 μM) for 30 minutes. The cells were treated with 0.1% DMSO (A), 0.5 mM POH (B) 0.1% DMSO plus JNK inhibitor V (C), and 0.5 mM POH plus JNK inhibitor V (D). After 24 hours of incubation, the cells were stained with annexin V-FITC and propidium iodide and analyzed by flow cytometry. Figure 7E represents the percentage of dead cells as indicated by early apoptosis and late apoptosis or necrosis (right lower quadrant + right upper quadrant, respectively), which was calculated from the data shown in Figures 7A-D. The data were expressed as the means ± SD from at least three different experiments. ***p < 0.001 vs. control group (0.1% DMSO), **p < 0.01 vs. control group (0.1% DMSO + JNK inhibitor V). The difference between the treated group (0.5 mM POH + JNK inhibitor V) and the control group (0.5 mM POH without the JNK inhibitor V) was not statistically significant. The data were analyzed by Student’s t-test.