Fig. 2

Effect of tectochrysin on the expression of apoptosis regulatory proteins and death receptors, and activation NF-κB in colon cancer cells. The colon cancer cells were treated with different concentrations of tectochrysin (1, 5 and 10 μg/mL) for 24 h or for 1 h (for NF-κB assay). Equal amounts of total proteins (20 μg/lane) were subjected to 10 % or 15 % SDS-PAGE. a Expression of cIAP1, XIAP, Bax, Bcl-2, Bid, Cyto-C, cleaved caspase-3, -8, -9, cleaved PARP and β-actin was detected by Western Blotting using specific antibodies. b Expression of DR3, DR4, Fas, and β−actin was detected by Western blotting using specific antibodies. c Activation of NF-κB was investigated using EMSA as described in Methods. After, the cells were treated with different concentrations (1, 5 and 10 μg/mL) of tectochrysin at 37 °C for 1 h, nuclear or cytosolic proteins were extracted and the expression of p50, p65, IκB-α, and p-IκB-α proteins was detected by Western blotting using specific antibodies. β-actin and histone-H1 proteins were used as internal controls. Each band is representative for three experiments. Values below the band are average of band density. (CE: cytosolic extraction, NE: nuclear extraction)