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Fig. 5 | Molecular Cancer

Fig. 5

From: Anticancer effect of tectochrysin in colon cancer cell via suppression of NF-kappaB activity and enhancement of death receptor expression

Fig. 5

Effect of tectochrysin on cell growth, DR4 and cleaved caspase-3 expression, and NF-κB activity in TRAIL-resistant cancer cell. a HT-29, A549, and MCF-7 cells were pretreated with tectochrysin (3 μg/mL) for 24 h, the media were removed, and the cells were exposed to TRAIL (50 ng/mL in HT-29, A549, and MCF-7 cells) for 24 h. The effect of tectochrysin on resistant cancer cell growth was determined by MTT assay. The data are expressed as the mean ± SD of three experiments. *P < 0.05 compared with the control. #P < 0.05 compared with the treated TARIL treated cells. b Expression of DR4, p-IκB, IκB, p50, β-actin and histone-H1 was detected by Western blotting using specific antibodies. β-actin and histone-H1 proteins were used as internal controls. c The activation of NF-κB was investigated using EMSA as described in Methods. Each band is representative for three experiments. Values below the band are average of band density

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