Fig. 6

Effect of tectochrysin on the tumor growth in HCT116 xenografts in vivo model. HCT116 xenografts mice were i.p treated with tectochrysin (5 mg/kg twice a week) for 3 weeks. a Tumor images (left, vehicle; right, tectochrysin 5 mg/kg), weights and volumes. The data are expressed as the mean ± SD of ten mice. *P < 0.05 compared with the vehicle mice. b Tumor extracts were analyzed by Western boltting. Samples (20 μg/lane) were resolved on 10 % or 15 % SDS-PAGE and detected with antibodies against cleaved caspase-3, DR3, DR4, p50, p65, β-actin, and Histone-H1. β-actin and histone-H1 proteins were used as internal controls. c DNA binding activity of NF-κB was determined by EMSA in nuclear extracts from tumor tissue of mice, as described under Methods. d Immunohistochemistry was used to determine expression levels of H&E, PCNA, DR3, cleaved capase-3, and p50 in tumor tissue of mice as described in Methods. Each figures represent for three animals, and each band is representative for three experiments. Values below the band are average of band density