Skip to main content
Fig. 1 | Molecular Cancer

Fig. 1

From: NOX1 to NOX2 switch deactivates AMPK and induces invasive phenotype in colon cancer cells through overexpression of MMP-7

Fig. 1

TPA-induced increase in MMP-7 expression corresponds to invasive ability of colorectal cancer cells. a Total RNAs from untreated basal HT29 and SW620 cells were analyzed for MMPs by qRT-PCR. The bar graph represents relative expression density of each MMP, and data values are the mean ± SEM from three independent experiments. *P < 0.05 compared to HT29 cells. b HT29 and SW620 cells seeded in a Matrigel-coated transwell were treated with TPA in serum-free media, and invaded cells were counted. The bar graphs represent the relative numbers of invaded cells. *P < 0.05 compared to vehicle-treated control group. # P < 0.05 compared to TPA-treated HT29 cells. c HT29 and SW620 cells were treated with TPA (12 ng/ml) for the indicated time, and extracted mRNAs and proteins were analyzed for induction of MMP-7 expression by qRT-PCR and immunoblotting. *p < 0.05 compared to vehicle-treated control group of each cell line. d Efficiencies of MMP-7 siRNA as measured by Western blotting in HT29 and SW620 cells. e In both HT29 and SW620 cells transfected with non-target (NT) and MMP-7 siRNAs, TPA-induced invasion was measured as described in (b). *P < 0.05 compared to vehicle-treated control (Mock) group. # P < 0.05 compared to NT siRNA-treated cells. All measurements were performed by three independent experiments. f, g TPA induced invasive phenotype change (f) in along with enhanced expression of MMP-7 (g) in Caco-2 and HCT116 cells

Back to article page