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Fig. 3 | Molecular Cancer

Fig. 3

From: NOX1 to NOX2 switch deactivates AMPK and induces invasive phenotype in colon cancer cells through overexpression of MMP-7

Fig. 3

Expression of NOX2 and its regulator subunits, p47phox and p67-phox, is regulated by NOX2-derived ROS in TPA-treated HT29 cells. a-b HT29 cells treated with TPA (12 ng/ml) for the indicated time periods were analyzed for gene expression by conventional PCR (a), qRT-PCR (b), and Western blotting (c). d Protein extracts from HT29 cells treated with DPI, NAC, and Vit. E in the presence of TPA were immunoblotted with anti-NOX2, p47phox, and p67-phox antibodies. β-Actin was used for equal loading. e HT29 cells were stimulated with H2O2 (5 μM) for the indicated time, and Western blotting was performed for detection of MMP-7, NOX2, p47phox, and p67-phox. Bands are representative of three independent experiments, and bar graphs indicate the mean ± SEM of relative densities of the gene products. *P < 0.05 compared to vehicle-treated control group. # P < 0.05 compared to TPA-treated group

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