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Fig. 6 | Molecular Cancer

Fig. 6

From: NOX1 to NOX2 switch deactivates AMPK and induces invasive phenotype in colon cancer cells through overexpression of MMP-7

Fig. 6

TPA-induced reduction of AMPK phosphorylation is dependent on NOX2-derived ROS production. a Protein extracts from HT29 cells pretreated with DPI (10 μM), NAC (10 μM), and Vit. E (50 μg/mL) for 1 h prior to treatment with TPA (1 h) were examined for phospho-AMPK/AMPK levels. b HT29 and SW620 cells transfected with siRNA specific to NT, NOX2, or p67phox were treated with TPA and analyzed for expression of phospho-AMPK. c HT29 cells were treated with exogenous H2O2 at the indicated concentrations for 1 h, and cell extracts were examined for levels of phospho-AMPK/AMPK, NOX1, NOX2, and MMP-7. d HT29 cells treated with H2O2 were analyzed for cellular ATP level using a Mitochondrial ToxGlo™ assay kit. Cytotoxicity was measured at the same time using the kit. (e, f) HT29 cells treated with AICAR or D942 in the presence or absence of TPA were analyzed for mRNA (e) or protein (f) expression. The bar and line graphs show the mean ± SEM from three independent experiments. *p < 0.05 compared to control, # p < 0.05 compared to TPA-treated group

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