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Fig. 3 | Molecular Cancer

Fig. 3

From: Kallikrein-related peptidase 6 regulates epithelial-to-mesenchymal transition and serves as prognostic biomarker for head and neck squamous cell carcinoma patients

Fig. 3

Silencing of KLK6 expression induces an EMT-like phenotype. (A) Western blot analysis with whole cell lysate of FaDu-Mock and FaDu-shKLK6 clones demonstrates differences in E-cadherin and Vimentin protein levels. Detection of β-Actin serves as control for quantity and quality of protein lysates. (B) Representative pictures of an IF staining confirms Vimentin expression (green signal) in FaDu-shKLK6 but not in of FaDU-Mock clones. Nuclear staining was done with Hoechst 33324 (blue staining); white bar = 20 μm. (C) Relative transcript levels of the indicated genes was assessed by quantitative RT-PCR as described above and depicted as mean value + SD of three independent FaDu-Mock and FaDu-shKLK6 clones. *p-value < 0.05, **p-value < 0.005, ***p-value < 0.0005

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