Fig. 4

mAb β4 treatment effect on BMD122-induced apoptosis in A431 cells by DNA laddering assay. (a) Comparison of A431 12-LOX transfectants with empty vector control (3.1+). Cells were treated with BMD122 (formerly BHPP) at the concentration indicated for 24 h, low molecular weight DNA was extracted, run on a 1.5 % agarose gel and visualized with ethidium bromide. The middle lanes are DNA markers for comparison. 3.1+: empty vector controls; 12LOX: A431 cells transfected with full-length 12-LOX. (b) A431 cells were pretreated with mAb β4 3E1 (5 μg/ml) before incubation in DMEM in the presence of BMD122, see details in Materials and Methods. Aliquots of DNA extracts were subjected to 1.5 % agarose gel and visualized with ethidium bromide. C: Ethanol as vehicle control. M: DNA marker, left lane