Fig. 9

Losartan-mediated effects on RGD4C.AAVP transduction. 9L cells were incubated overnight with increasing concentrations 20 μM, 50 μM, 100 μM, 150 μM, 200 μM of losartan and transduced the following day with targeted RGD4C.AAVP or non-targeted NT.AAVP vectors expressing the Luc gene. Luc gene expression was quantified 3 days post-transduction by the luciferase Steady-Glo® assay kit. Means from triplicates are shown while the graph is representative of similar experiments (n = 2). One way ANOVA with application of Tukey’s post-test, *, p < 0.05, was performed to determine conditions for maximal transduction success rate. Data are presented as mean ± s.e.m. Experiments were repeated twice and individual conditions were repeated in triplicates