Fig. 7

Human NK cell analysis. (a) Kidneys were excised from mice one day after antibody injection and examined for human NK cell influx into the engrafted tumor by immmunofluorescent analysis of human NK cells (CD56⁺, red) and contrasted against DAPI (blue). Data are from the IgG1 and G37 treatment groups, and representative of control and anti-CAIX treatments, respectively. (b) Human NK cell populations were analyzed by FACS after gating on human CD45⁺CD3⁻ cells in the peripheral blood of mice at day 11 and 17 from mice in the G37 treatment group, (c) The ADCC of freshly isolated human PBMC (left, hu-PBMC) and PBMC isolated from NSG mice 32 days after human PBMC injection from the same donor (hu-NSG PBMC, right) were determined against RCC cells used to establish orthotopic tumors with control or G37 IgG1. Data represent means of three measurements. ** indicates p value of student t-test < 0.01, compared to control IgG1