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Fig. 1 | Molecular Cancer

Fig. 1

From: Alterations in microRNAs miR-21 and let-7a correlate with aberrant STAT3 signaling and downstream effects during cervical carcinogenesis

Fig. 1

Expression levels of miR-21 and let-7a in cervical cancer cells. a & b. Representative photograph showing specific amplification of miR-21 and let-7a in quantitative real-time RT-PCR (qRT-PCR) of total RNA isolated from cervical cancer lines, C33a (HPV-), SiHa & CaSki (HPV16+) and HeLa (HPV18+) (a); or from cervical tissues of normal (N), low grade squamous intra-epithelial lesion (LSIL), high grade SIL (HSIL) and cervical cancer tissues (C1, C2) (b). Amplification of U6 RNA was performed as internal control for input RNA. miR fold change was calculated w.r.t. C33a in case of cell lines or reference pooled RNA from normal tissues. Marker, ΦX174 Hae III-digested molecular weight marker; N, PCR negative control. c. Cumulative data showing mean ± S.E. of fold change expression of miR-21 and let-7a in tumor tissues of pre-cancer and cancer lesions. d & e. Distribution of miR-21 and let-7a expression in cancer tissues of different clinical stages (d) or in different histopathological grades (e) data showing mean ± S.E. of fold change expression of miR-21 and let-7a in tumor tissues examined in 3 independent experiments. *p value <0.05; **p value <0.01; ***p value <0.001. Note: The fold change being reflected in a & b may not corroborate with c, d & e as they are end products of the qRT-PCR run

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