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Fig. 2 | Molecular Cancer

Fig. 2

From: Alterations in microRNAs miR-21 and let-7a correlate with aberrant STAT3 signaling and downstream effects during cervical carcinogenesis

Fig. 2

Association of miR-21 and let-7a with STAT3 expression and activation. a & b. Representative immunoblots showing expression of total STAT3 and pSTAT3 (Y705) in cervical cancer cell lines (a) and in cervical tissues (b). Total cellular proteins (50 μg) isolated from indicated cell lines and cervical tissues were immunoblotted for STAT3, pSTAT3 (Y705) and β-actin expression by respective antibodies. Values below each blot represent relative difference in levels of indicated proteins w.r.t. normal. Normalized fold difference in expression of STAT3/pSTAT3 band in each tissue type was determined by densitometry as described in Methods in 3 independent experiments. c & d. Distribution of miR-21 (c) and let-7a (d) levels (Mean ± SE) in cervical tissues from pre-cancer and cancer lesions with corresponding STAT3/pSTAT3 protein levels. STAT3/pSTAT3 levels were broadly classified into low or high STAT3/pSTAT3 based on median fold difference in levels of expression of respective protein in each disease group. STAT3 median values were LSIL – 0.97; HSIL – 1.27; Cancer – 2.1. pSTAT3 median values were LSIL – 0.68; HSIL – 1.06; Cancer – 1.51. *p value <0.05; **p value <0.01; ***p value <0.001

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