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Fig. 3 | Molecular Cancer

Fig. 3

From: Alterations in microRNAs miR-21 and let-7a correlate with aberrant STAT3 signaling and downstream effects during cervical carcinogenesis

Fig. 3

Association of miR-21 and let-7a with HPV16 E6 expression. a & b. Representative immunoblots showing levels of HPV16 E6 in indicated cervical cancer cell lines (a) and biopsy tissues (b). β-actin levels were used as input control. Normalized fold difference in expression of HPV16 E6 band in each tissue type was determined by densitometry as described in Methods. c & d. Distribution of miR-21 (c) and let-7a (d) levels (Mean ± SE) in HPV16-positive cervical tissues from pre-cancer and cancer lesions with corresponding E6 protein levels. Relative normalized E6 levels were broadly classified into low or high groups based on median fold difference in HPV16 E6 levels in each disease group. Fold change in E6 expression was measured w.r.t. E6 levels in SiHa cells which were used as reference. HPV16 E6 protein level median values were LSIL – 0.91; HSIL – 0.98; Cancer – 1.94. *p value <0.05; **p value <0.01; ***p value <0.001

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